Hair Follicle Dermal Papilla Cells, HFDPC

Hair Follicle Dermal Papilla Cells (HFDPC)
Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchyme cells isolated from the hair papilla of normal human scalp hair follicles. They are cryopreserved at second passage, and can be cultured and propagated 6 population doublings. HFDPC can be used for the development and evaluation of hair growth products. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. It has been shown that cultured HFDPC can stimulate hair growth when implanted into an amputated follicle¹. When used in a reconstitution model, HFDPC is an excellent tool for identifying cell populations within the hair follicle and studying the effects of specific gene products on hair follicle growth and development². Jahoda, C. et al, J. Invest. Dermatol. 101:584 (1993). Weinberg, W. et al, J. Invest. Dermatol. 100: 229 (1993).
		Characterization: Positive for alkaline phosphatase. Negative for vWF antigen expression.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HFDPC Total Kit , adult	Each kit contains an ampoule of cryopreserved HFDPC (602-05a), 500 ml of HFDPC Growth Medium (611-500), a Subculture Reagent Kit, and 50 ml of Collagen Coating Solution (125-50).	1 Kit	602K-05a
Cryopreserved HFDPC , adult	(>500,000 cells in an ampoule) Cryopreserved at third passage in Papilla Cell Basal Medium that contains 20% FBS and 5% DMSO.	1 Ampoule	602-05a
Proliferating HFDPC , adult In Flasks	Cultured in flasks at fourth passage in Papilla Cell Growth Medium and shipped in Papilla Cell Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	603-25a 603-50a 603-75a
Proliferating HFDPC , adult In Multiwell Plates	Cultured in plates at fourth passage in Papilla Cell Growth Medium and shipped in Papilla Cell Transfer Medium.	6 well 12 well 24 well 48 well 96 well	603-6Wa 603-12Wa 603-24Wa 603-48Wa 603-96Wa
HFDPC Growth Medium	Optimized and ready for use	500 ml	611-500
HFDPC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	611K-500
HFDPC Basal Medium	Add Growth Supplement before use	500 ml	610-500
HFDPC Growth Supplement		30 ml	611-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
Collagen Coating Solution		50 ml	125-50
HFDPC RNA , adult	Total RNA prepared from HFDPC	10 µg 25 µg	602-R10a 602-R25a