Human Epidermal Melanocytes

(HEM)

Human Epidermal Melanocytes (HEM) are primary cells isolated from normal human neonatal foreskin. HEM are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. These epidermal melanocytes produce melanin, thus a useful cell model in the study of hyperpigmentation through accentuated melanocyte proliferation and differentiation^1,2, as well as progression of melanocytic neoplasia^3,4.

Jimbow, K. et al, J. Invest. Dermatol. 78:108 (1982).
lmokawa, G. et al, Arch. Dermatol. Res. 278:352 (1986).
Herlyn, M. et al, Lab. Invest. 56:461(1987)
Simon, H., Cancer Res. 56:3112 (1996).

Characterization:

Typical stellate cell morphology.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HEM Total Kit , neonatal	Each kit contains an ampoule of cryopreserved HEM (104-05), 500 ml of Melanocyte Cell Growth Medium (135-500) and a Subculture Reagent Kit (090K).	1 Kit	104K-05n
Cryopreserved HEM , neonatal	(>500,000 cells in an ampoule) Cryopreserved at second passage in Melanocyte Basal Medium that contains 30% FBS and 10% DMSO.	1 Ampoule	104-05n
Proliferating HEM , neonatal In Flasks	Cultured and shipped in flask at third passage in Melanocyte Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	105-25n 105-50n 105-75n
Proliferating HEM , neonatal In Multiwell Plates	Cultured and shipped in plates at third passage in Melanocyte Growth Medium.	6 well 12 well 24 well 48 well 96 well	105-6Wn 105-12Wn 105-24Wn 105-48Wn 105-96Wn
Melanocyte Growth Medium	Optimized and ready for use.	500 ml	135-500
Melanocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	135K-500
Melanocyte Basal Medium	Add Growth Supplement before use	500 ml	134-500
Melanocyte Growth Supplement		5 ml	135-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
Melanocyte RNA , neonatal	Total RNA prepared from HEM	10 µg 25 µg	104-R10n 104-R25n