Human Coronary Artery
Smooth Muscle Cells
(HCASMC)
|
|
Human
Coronary
Artery
Smooth
Muscle
Cells
(HCASMC)
are
derived
from
the
tunica
intima
and
tunica
media
of
normal
human,
fibrous
plaque-free
coronary
arteries.
They
are
cryopreserved
at
second
passage
and
can
be
cultured
and
propagated
at
least
16
population
doublings.
Smooth
muscle
cells
are
present
in
various
amounts
in
early
fatty
streaks
and
they
are
the
predominant
type
of
cells
in
fibrous
plaques1,2,3.
Atherosclerosis
of
the
coronary
arteries
can
cause
angina
pectoris
and
heart
attack.
Intimal
smooth
muscle
proliferation
plays
a
key
event
in
the
development
of
atherosclerosis.
HCASMC
is
an
ideal
cellular
model
for
the
investigations
of
coronary
artery
diseases,
such
as
alteration
of
smooth
muscle
cell
differentiation
in
atherosclerosis4,5.
-
Ross, R.N., Nature
362:801 (1993).
-
McGill,
H.C.
Jr.,
Arteriosclerosis
4:443
(1984).
-
Jonasson,
L.
et
al,
Artherosclerosis
6(2):131
(1986).
-
Owens.
G.K.,
Atherosclerosis
and
Coronary
Artery Disease
Chap3:401
(1996).
-
Fager,
G.
et
al,
In
Vitro
Cell.
Biol.
25(6):511
(1989).
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|
HCASMC
|
e2s.jpg)
Differentiated HCASMC
stained for smooth muscle specific α-actin
|
| Characterization:
Positive for smooth muscle cell specific a
-actin expression.
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|
Each
lot tested negative for HIV, Hepatitis B and Hepatitis C and negative
for mycoplasma, bacteria, yeast, fungi. |
|
Product Name
|
Product Description
|
Size (vol)
|
Cat. No.
|
| HCASMC Total Kit
, adult
|
Each kit contains an ampoule of cryopreserved HAOSMC(350-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.
|
1 Kit
|
350K-05a
|
| Cryopreserved HCASMC
, adult
|
(>500,000 cells in an ampoule)
Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.
|
1 Ampoule
|
350-05a
|
|
Proliferating HCASMC
, adult
In Flasks
|
Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.
|
T-25 Flask
2 T-25 Flasks
T-75 Flask or 3 T-25 Flasks
|
351-25a
351-50a
351-75a
|
|
Proliferating HCASMC
, adult
In Multiwell Plates
|
Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.
|
6 well
12 well
24 well
48 well
96 well
|
351-6Wa
351-12Wa
351-24Wa
351-48Wa
351-96Wa
|
| Smooth Muscle Cell Growth Medium
|
Optimized and ready for use.
|
500 ml
|
311-500
|
| Smooth Muscle Cell Growth Mediium Kitl
|
Basal Medium & Growth Supplement packaged separately.
|
Yields 500 ml
|
311K-500
|
| Smooth Muscle Cell Basal Medium
|
Add Growth Supplement before use.
|
500 ml
|
310-500
|
| Smooth Muscle Cell Growth Supplement
|
|
30 ml
|
311-GS
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| Smooth Muscle Cell Differentiation Medium
|
|
250 ml
|
311D-250
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| Subculture Reagent Kit
|
100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution
|
100 ml
|
090K
|
| HCASMC RNA
, adult
|
Total RNA prepared from HCASMC.
|
10 µg
25 µg
|
350-R10a
350-R25a
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