Human Aortic Smooth Muscle Cells, HAOSMC

Human Aortic Smooth Muscle Cells

(HAOSMC)

Human Aortic Smooth Muscle Cells (HAOSMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Arterial smooth muscle cells are capable of synthesizing collagen, elastin, myosin and glycosaminoglycan^1,2. Increased production of connective tissue components, hyperplasia and hypertrophy of intimal smooth muscle cells are found to gradually occlude the vessel lumen in atherosclerosis^1,2. HAOSMC respond to various factors by proliferating or differentiating^3,4. They are a well established cell system for the study of human vascular disorders such as atherosclerosis^5,6 and stroke.

Abraham, P.A. et al, Biochem. Biophys. Res. Comm. 58:597 (1974).
Ross, R., New Engl. J. Med. 314(8):488 (1986).
Fager, G. et al, In Vitro Cell. Biol. 25(6):511 (1989).
Hoshi, H. et al, In Vitro Cell. Biol. 24(4):309 (1988).
Orekhov, A.N. et al, Lab. Invest. 48:749 (1983).
Jonasson, L. et al, Arteriosclerosis 6(2):131 (1986).

Human aortic smooth muscle cells, HAOSMC

HAOSMC

Actin stained human aortic smooth muscle cells, HAOSMC

α-actin stain

Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HAOSMC Total Kit , adult	Each kit contains an ampoule of cryopreserved HAOSMC (354-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.	1 Kit	354K-05a
Cryopreserved HAOSMC , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	354-05a
Proliferating HAOSMC , adult In Flasks	Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	355-25a 355-50a 355-75a
Proliferating HAOSMC , adult In Multiwell Plates	Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	355-6Wa 355-12Wa 355-24Wa 355-48Wa 355-96Wa
Smooth Muscle Cell Growth Medium	Optimized and ready for use	500 ml	311-500
Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	311K-500
Smooth Muscle Cell Basal Medium	Add Growth Supplement before use	500 ml	310-500
Smooth Muscle Cell Growth Supplement		30 ml	311-GS
Smooth Muscle Cell Differentiation Medium		250 ml	311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HAOSMC RNA , adult	Total RNA prepared from HAOSMC	10 µg 25 µg	354-R10a 354-R25a