Human Dermal Microvascular Endothelial Cells, HMVEC CADMEC

Human Dermal Microvascular Endothelial Cells (HMVEC/CADMEC™)
Cell Applications' Dermal Microvascular Endothelial Cells (CADMEC™) are isolated from normal human neonatal foreskin or adult skin capillaries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They are responsive to cytokine stimulation in the expression of cell adhesion molecules. CADMEC™ express CD36 constitutively, while large vessel endothelial cells do not. CADMEC™ is an organ-specific endothelial cell system for the study of angiogenesis¹, tumor metastasis^2,3, wound healing⁴, inflammation^5,6, macromolecule transport⁷, and drug metabolism⁸. There is growing evidence of differences in physiology and pharmacology between microvascular endothelial cells and macrovascular endothelial cells^9,10. Swerlick, R.A. et.al, J. Invest. Dermatol. 97(2):190 (1991) Folkman, J. et al, Nature 288:551 (1980). Lee, K.H. et al, J. Invest. Dermatol. 98(1):79 (1992). Deryugina, E.I. et al, Hybridoma 15(4):279 (1996) Sepp, N.T. et al, J. Invest. Dermatol. 104(2):277 (1995). Swerlick, R.A. et al, J. Invest. Dermatol. 100(1):111S (1993). Hanasaki, K. et al, J. Bio. Chem. 270(13):7533 (1995) Pruckler, J.M. et al, Pathobiology 61:283 (1993). Foster, C.A. et al, J. Dermatol. 21(11):847 (1994). Schnitzer, J.E. et al, BBRC. 199(1):11 (1994).	HMVEC/CADMEC	Cytokine Stimulated Leukocyte adhesion.
	Characterization: Positive for Factor VIII-related antigen expression. Positive for Factor VIII-related Dil-Ac-LDL uptake. Positive for CD36 expression. Positive for functional activity by cytokine-stimulated leukocyte adherence.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi. We have special lots that were verified to be responsive to VEGF. Please call to discuss how these lots can meet your needs. The activation of VEGF-R2 was validated by autophosphorylation at tyrosine 1175. VEGF-R2 is a high affinity receptor for VEGF whcih stimulates proliferation, chemotaxis and sprouting of endothelial cells in vitro and angiogenesis in vivo . via VEGF-R2 signalling pathway. click here for Pre-Screened HUVEC and CADMEC Cells...

Product Name	Product Description	Size (vol)	Cat. No.
CADMEC™ Total Kit adult neonatal	Each kit contains an ampoule of cryopreserved CADMEC™ (100-05), 500 ml of CADMEC™ Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100).	1 Kit	100K-05a 100K-05n
Cryopreserved CADMEC™ adult neonatal	(>500,000 cells in an ampoule) Cryopreserved at second passage in CADMEC™ Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	100-05a 100-05n
Proliferating CADMEC™ In Flasks *Specify adult (a) or neonatal (n)	Cultured in flasks at third passage in CADMEC™ Growth Medium and shipped in Endothelial Cell Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	101-25a; 101-25n * 101-50a; 101-50n * 101-75a; 101-75n *
Proliferating CADMEC™ In Multiwell Plates *Specify adult (a) or neonatal (n)	Cultured in plates at third passage in CADMEC™ Growth Medium and shipped in Endothelial Cell Transfer Medium.	6 well 12 well 24 well 48 well 96 well	101-6Wa; 101-6Wn * 101-12Wa; 101-12Wn * 101-24Wa; 101-24Wn * 101-48Wa; 101-48Wn * 101-96Wa; 101-96Wn *
CADMEC™ Growth Medium	Optimized and ready for use	500 ml	112-500
CADMEC™ Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	112K-500
CADMEC™ Basal Medium	Add Growth Supplement before use	500 ml	110-500
CADMEC™ Growth Supplement		30 ml	112-GS
Attachment Factor Solution	Promotes attachment of endothelial cells.	100 ml	123-100
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
CADMEC™ RNA adult (a) neonatal (n) Stimulated CADMEC™ Total RNA adult (a) neonatal (n)	Total RNA prepared from CADMEC™ Total RNA prepared from stimulated CADMEC™	10 µg / 25 µg 10 µg / 25 µg	100-R10a; 100-R25a 100-R10n; 100-R25n 100S-R10a; 100S-R25a 100S-R10n; 100S-25n

Human Dermal Microvascular Endothelial Cells

(HMVEC/CADMEC™)