Feline Osteoblasts

(FOb)

Feline Osteoblasts (FOb) are isolated from feline bone and are cryopreserved at second passage. FOb can be passaged at least 10 population doublings. The feline model provides a system for the study of bone-remodeling agents^1,2,3,4. Canine osteoblasts have been used in bone regeneration models that utilize scaffolds such as 3-D bioactive polycaprolactone⁵.

1. Davidovitch, Z et al., Am J Orthod. 77(1):14 (1980).

2. Davidovitch, Z e al., Calcif Tissue Int. 36(S1):S86 (1984).

3. Davidovitch, Z et al., Prog Clin Biol Res. 101:125 (1982).

4. Brin, I et al., Am J Orth. 79(2):162 (1981).

5. Davidovitch, Z et al., Calcif Tissue Res. 24(1):81-91 (1977).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
FOb Total Kit	Each kit contains an ampoule of cryopreserved FOb (F406-05), 500 ml of Feline Osteoblast Growth Medium (F417-500), and a Subculture Reagent Kit (090K).	1 kit	F406K-05
Cryopreserved FOb	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Feline Osteoblast Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	F406-05
Proliferating FOb	Cultured in flasks at second passage in Feline Osteoblast Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	F407-25 F407-50 F407-75
Proliferating FOb	Cultured in plates at second passage in Feline Osteoblast Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	F407-6w F407-12w F407-24w F407-48w F407-96w
Feline Osteoblast Growth Medium	Optimized and ready for use.	500 ml	F417-500
Feline Osteoblast Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	F417K-500
Feline Osteoblast Basal Medium	Add Growth Supplements before use.	500 ml	F416-500
Feline Osteoblast Growth Supplements		50 ml	F417-GS
Feline Osteoblast Differentiation Medium		250 ml	F417D-250
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K