Feline Chondrocytes

(FC)

Feline Chondrocytes (FC) are derived from the articular cartilage of cats and are cryopreserved at second passage. FC can be passaged at least 10 population doublings. Following monolayer culturing, FC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system¹. Alginate microspheres allow chondrocytes to produce and retain surrounding proteoglycans as in articular cartilage. Because of the prevalence of degenerative joint disease (DJD) in geriatric cats², FC can be a useful tool for veterinary practice.

1. Kamishina, H et al., J Vet Med Sci. 68(11):1239 (2006).

2. Hardie, EM et al., J Am Vet Med Assoc. 220(5):628 (2002).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
FC Total Kit	Each kit contains an ampoule of cryopreserved FC (F402-05), 500 ml of Feline Chondrocyte Growth Medium (F411-500), and a Subculture Reagent Kit (090K).	1 kit	F402K-05
Cryopreserved FC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Feline Chondrocyte Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	F402-05
Proliferating FC	Cultured and shipped in flasks at second passage in Feline Chondrocyte Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	F403-25 F403-50 F403-75
Proliferating FC	Cultured and shipped in plates at second passage in Feline Chondrocyte Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	F403-6W F403-12W F403-24W F403-48W F403-96W
Feline Chondrocyte Growth Medium	Optimized and ready for use.	500 ml	F411-500
Feline Chondrocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	F411K-500
Feline Chondrocyte Basal Medium	Add Growth Supplements before use.	500 ml	F410-500
Feline Chondrocyte Growth Supplements		50 ml	F411-GS
Feline Chondrocyte Differentiation Medium		250 ml	F411D-250
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K