Canine Pulmonary Artery Smooth Muscle Cells

(CnPASMC)

Canine Pulmonary Artery Smooth Muscle Cells (CnPASMC) are derived from the tunica intima and tunica media of canine pulmonary arteries and are cryopreserved at second passage. CnAOEC can be passaged at least 16 population doublings. The canine model has been used to study the role of calcium entry^{1,2,3,4,5,6,7}, ClC-3 channels^8,9, PDGF^10,11, and PKC^12,13 on pulmonary artery smooth muscle cell function.

1. Shimizu, S et al., Anesthesiology. 104(4):791 (2006).

2. Ng, LC et al., J Physiol. 563(2):409 (2004).

3. Horibe, M et al., Anesthesiology. 95(3):681 (2001).

4. Doi, S et al., Am J Physiolo Lung Cell Mol Physiol. 279(2):L242 (2000).

5. Hong, SJ et al., Anesthesiology. (88)3:792 (1998).

6. Hamada, H et al., Circ Res. 81(5):812 (1997).

7. Hamada, H et al., Anesthesiology. 87(4):900 (1997).

8. Dai, YP et al., Br J Pharmacol. 145(1):5 (2005).

9. Wang, GX et al., Am J Physiol Heart Circ Physiol. 285(4):H1453 (2003).

10. Bongalon, S et al., J Vas Res. 41(5):412 (2004).

11. Yamboliev, IA & Gerthoffer, WT., Am J Physiol Cell Physiol. 280(6):

C1680 (2001).

12. Zhong, J et al., Am J Physiol Cell Physiol. 283(6):C1627 (2002).

13. Damron, DS et al., Am J Physiol. 274(2pt1):L278 (1998).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
CnPASMC Total Kit	Each kit contains an ampoule of cryopreserved CnPASMC (Cn352-05), 500 ml of Canine Smooth Muscle Cell Growth Medium (Cn311-500), and a Subculture Reagent Kit (090K).	1 kit	Cn352K-05
Cryopreserved CnPASMC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	Cn352-05
Proliferating CnPASMC	Cultured in flasks at second passage in Canine Smooth Muscle Cell Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	Cn353-25 Cn353-50 Cn353-75
Proliferating CnPASMC	Cultured in plates at second passage in Canine Smooth Muscle Cell Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	Cn353-6w Cn353-12w Cn353-24w Cn353-48w Cn353-96w
Canine Smooth Muscle Cell Growth Medium	Optimized and ready for use.	500 ml	Cn311-500
Canine Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	Cn311K-500
Canine Smooth Muscle Cell Medium	Add Growth Supplements before use.	500 ml	Cn310-500
Canine Smooth Muscle Cell Growth Supplements		50 ml	Cn311-GS
Canine Smooth Muscle Cell Differentiation Medium		250 ml	Cn311D-250
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K