Canine Aortic Smooth Muscle Cells

Canine Aortic Smooth Muscle Cells

(CnAOSMC)

Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings. Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in intimal hyperplasia and atherosclerosis. It was shown that significant regional difference in PDGF production in normal canine aorta, and that SMC are a significant contributor to the regional variation in PDGF production¹. It was also shown that nebivolol relaxes vascular smooth muscle by NO- and cyclic GMP-dependent mechanisms². Graft SMCs are functionally altered producing more platelet-derived growth factor (PDGF) than aortic SMCs. PDGF produced by graft SMCs may contribute to the development of intimal hyperplasia³. Graft SMCs have decreased proliferation response, but have similar migratory response to PDGF compared with aortic SMCs⁴. Intrinsic differences in endothelial cells from proximal aorta versus the distal aorta have different capacity to produce PDGF in response to stimulants whereas unstimulated SMCs did not exhibit regional variation in PDGF production and did not increase PDGF secretion after PMA or thrombin teatment⁵. Identification of the cDNA for canine isoform of nitric oxide synthase (iNOS) can be used in the study of allograft rejection and cardiovascular disease⁶.

Ignarro, L.J. et al, Nitric Oxide. 7(2):75-82 (2002).
Madura, J.A. et al, J. Vas Res. 33(1):53-61 (1996).
Pitsch, R.J. et al, J. Vasc. Surg. 26(1):70-8 (1997).
Minion, D.J. et al, J. Vasc. Surg. 31(5):953-9 (2000).
Van Aalst, J.A. et al, J. Vasc. Surg. 32(3):584-92 (2000).
Wang, X. et al, Am. J. Physiol. 275(4 Pt 2):H1122-9 (1998).

Animal Cells - Canine Aortic Smooth Muscle Cells

Characterization: Positive for smooth muscle specific α-actin expression.

Product Name	Product Description	Size (vol)	Cat. No.
CnAOSMC Total Kit	Each kit contains an ampoule of cryopreserved CnAOSMC (Cn354-05), 500 ml of Canine SMC Growth Medium (Cn311-500), and a Subculture Reagent Kit.	1 Kit	Cn354K-05
Cryopreserved CnAOSMC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Canine SMC Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	Cn354-05
Proliferating CnAOSMC In Flasks	Cultured and shipped in flasks at third passage in Canine SMC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	Cn355-25 Cn355-50 Cn355-75
Proliferating CnAOSMC In Multiwell Plates	Cultured and shipped in plates at third passage in Canine SMC Growth Medium.	6 well 12 well 24 well 48 well 96 well	Cn355-6W Cn355-12W Cn355-24W Cn355-48W Cn355-96W
Canine SMC Growth Medium	Optimized and ready for use	500 ml	Cn311-500
Canine SMC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	Cn311K-500
Canine SMC Basal Medium	Add Growth Supplement before use	500 ml	Cn310-500
Canine SMC Growth Supplement		50 ml	Cn311-GS
Canine SMC Differentiation Medium		250 ml	Cn311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K