Canine Aortic Endothelial Cells

(CnAOEC)

Canine Aortic Endothelial Cells (CnAOEC) are endothelial cells isolated from the dog aorta. CnAOEC are cryopreserved at second passage and can be passaged at least 16 doublings. The dog model provides an in vitro system to study cardiovascular diseases such as aortic aneurysms¹. The aorta of the canine is commonly used as a model for stent-graft testing for the aneurysm treatment^2,3^,4. Studies have shown that cultured canine aortic endothelial cells express cardiotrophin-1 (CT-1), glycoprotein (gp)130, and the vasoconstricting and mitogenic peptide endothelin-1 (ET-1)⁵. Cultured CnAOEC have also been shown to release vasodilatory products in response to acetylcholine and adenosine⁶.

1. Milne, AA et al., Br. J Surg. 82(5):635 (1995).

2. Weon, YC et al., AJR Am J Roentgenol 186(4):1148 (2006).

3. Castaneda, F et al., Cardiovasc Intervent Radiol. 23(5):375 (2000).

4. Konya, A & Wright, KC. Acta Radiol. 41(1):67 (2000).

5. Jougasaki, M et al., Peptides. 23(8):1441 (2002).

6. Woodley, N & Barclay, JK. Can J Physiol Pharmacol 74(8):949 (1996).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat No.
CnAOEC Total Kit	Each kit contains an ampoule of cryopreserved CnAOEC (Cn304-05), 500 ml of Canine Endothelial Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K).	1 kit	Cn304K-05
Cryopreserved CnAOEC	(>500,000 cells in an ampoule) Cryopreserved in ampoules at first passage in Canine Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 ampoule	Cn304-05
Proliferating CnAOEC	Cultured in flasks at second passage in Canine Endothelial Cell Growth Medium.	T-25 flask 2 T-25 flasks T-75 flask or 3 T-25 flasks	Cn305-25 Cn305-50 Cn305-75
Proliferating CnAOEC	Cultured in plates at second passage in Canine Endothelial Cell Growth Medium.	6 well plate 12 well plate 24 well plate 48 well plate 96 well plate	Cn305-6w Cn305-12w Cn305-24w Cn305-48w Cn305-96w
Canine Endothelial Cell Growth Medium	Optimized and ready for use.	500 ml	Cn211-500
Canine Endothelial Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500ml	Cn211K-500
Canine Endothelial Cell Basal Medium	Add Growth Supplements before use.	500 ml	Cn210-500
Canine Endothelial Cell Growth Supplements		30 ml	Cn211-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)	100 ml	090K