| Cat.# |
CB5861
|
| Size |
90 µg / 200
ml |
| Isotype:
|
rabbit polyclonal
IgG
|
| Epitope: |
E.
coli-expressed
cytoplasmic domain of the human insulin receptor-b
subunit.
|
|
Species
&
specficity:
|
Human,
mouse, & rat Insulin Receptor-b.
Anti-Insulin
Receptor-b
specifically detects endogenous levels of human insulin
receptor-b.
This
antibody does not cross-react with other IR-family members.
|
| Storage: |
Store
at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw
cycles.
|
| MW:
|
97
kDa
|
|
Application:
|
IHC
(Paraffin)
|
IP |
WB
|
FACS |
| Dilution:
|
1:50 |
1:50 |
1:1000
|
1:50 |
|
Background:
Insulin receptor is a transmembrane
receptor that is activated by insulin. It belongs to the large class of
tyrosine kinase receptors. Two alpha subunits and two beta subunits make
up the insulin receptor. The beta subunits pass through the cellular
membrane and are linked by disulfide bonds.1 Insulin receptor
functions as an enzyme that transfers phosphate groups from ATP to
tyrosine residues on intracellular target proteins. Binding of insulin
to the alpha subunits causes the beta subunits to phosphorylate
themselves (autophosphorylation), thus activating the catalytic activity
of the receptor. The activated receptor then phosphorylates a number of
intracellular proteins, which in turn alters their activity, thereby
generating a biological response.2
Several
intracellular proteins have been identified as phosphorylation
substrates for the insulin receptor, the best studied of which is insulin
receptor substrate 1 or IRS-1. When IRS-1 is activated by
phosphorylation, it serves as a type of docking center for recruitment
and activation of other enzymes that ultimately mediate insulin's
effects, which include activation of PI-3-kinase and MAP kinase pathway
etc.3,4
References:
1.
Youngren, J.F.: Cell
Mol Life Sci. 64:873, 2007.
2. Backer,
J.M. et al. J
Cell Biol.118:831, 1992.
3. Sesti, G. : Best
Pract Res Clin Endocrinol Metab. 20:665, 2006.
4. Gual, P. et al. :
Biochimie 87:99, 2005
.
|
|
|
