Elk1: Monoclonal Elk1 Antibody (18A1)

Background: Elk1 is a member of ETS-domain transcription factor family. ETS proteins form one of the largest families of signal-dependent transcriptional regulators, mediating cellular proliferation, differentiation and tumorigenesis¹. Elk-1 is regulated by phosphorylation in response to activation of the MAPK (mitogen-activated protein kinase) pathways. This phosphorylation triggers a series of molecular events that convert Elk-1 from a transcriptionally silent state into a highly active state and then back to a basal level². At the same time, activation of the ERK (extracellular-signal-regulated kinase) MAPK pathway leads to loss of modification of Elk-1 by SUMO (small ubiquitin-related modifier). As SUMO imparts repressive properties on Elk-1, ERK-mediated SUMO loss leads to de-repression at the same time as the ERK pathway promotes activation of Elk-1³. Thus a two-step mechanism is employed to convert Elk-1 into its fully activated state.

References:

1. Tsu, T. et al.: J. Cell. Biochem. 91:896, 2004.

2. Yang, S. H. & Sharrocks, A. D.: Biochem. Soc. Symp. 73:121, 2006.

3. Yang, S. H. et al.: Mol. Cell 12:63, 2003.

Specific detection of Elk1 protein in K562 cell lysate by Western blot analysis using Elk1 Monoclonal Antibody (18A1).