Lck: Monoclonal Anti-Lck Antibody (19D8)

Background: Lck (lymphocyte cellular kinase) is a member of the Src family of protein tyrosine kinases, that plays a key role in T-lymphocyte activation and differentiation. It is associated with a variety of cell surface receptors and is critical for signal transduction from the T-cell antigen receptor (TCR).¹ In many non-lymphoid cells, Lck plays an important role in mediating the signaling from  cell surface receptor tyrosine kinase to downstream components, e.g.  Lck is required for c-Kit-stimulated MAK kinase activation in lung cancer cells.² In some cancer cells, p56lck could favor metastases by facilitating loss of cell adhesion.³ Under certain conditions Lck is also involved in the induction of apoptosis.⁴ In addition, Lck is targeted by regulatory proteins of T-lymphotropic viruses, especially by the Herpesvirus saimiri (HVS) tyrosine kinase interacting protein (Tip). This oncoprotein physically interacts with Lck in HVS transformed T cells and has an impact on its catalytic activity.⁵ Understanding the regulation of Lck activity may serve as a basis for the development of new drugs capable of modifying Lck activity in different pathological situations.

References:

1. Palacios, E.H. & Weiss, A. : Oncogene 23:7990, 2004.

2. Bondzi, G. et al.  : Cell Growth Differ. 11:305, 2000.

3. Rouer, E. : Bull. Cancer 91:928, 2004.

4. Samraj, A.K. et al. : Oncogene 25:186, 2006.

5. Isakov, N. & Biesinger, B. : Eur. J. Biochem. 267:3413, 2000.

Specific detection of Lck proteins from Jurkat cell lysate in Western blot analysis using Lck Monoclonal Antibody (19D8).