| Cat.# |
CB12470
|
| Size |
300
µg / 200
ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
NSO-expressed
recombinant human BMP-4 protein |
|
Species
&
specficity:
|
Human
& mouse BMP-4 proteins. Anti-BMP-4 does not cross-react
with other BMP family members.
|
| Storage: |
-20°
|
| MW:
|
47
kDa
|
|
Application:
|
IHC
(Paraffin)
|
WB
|
IP |
| Dilution:
|
1:50 |
1:1000
|
1:50 |
|
Background:
Bone
morphogenetic protein 4 (BMP-4) is a polypeptide belonging to the TGF-bTGF
superfamily of proteins. Like other bone morphogenetic proteins, BMP-4
is involved in bone and cartilage development, specifically tooth and
limb development and fracture repair. It has been shown to be involved
in muscle development, bone mineralization, and uteric bud development1.
In human embryonic development, BMP-4 is a critical signaling molecule
required for the early differentiation of the embryo and establishment
of a dorsal-ventral axis. BMP-4 is secreted from the dorsal portion of
the notochord, and it acts in concert with sonic hedgehog (released from
the ventral portion of the notochord) to establish a dorsal-ventral axis
for the differentiation of later structures2. BMP-4
stimulates differentiation of overlying ectodermal tissue3.
Inhibition of the BMP-4 signal (by chordin, noggin, or follistatin)
causes the ectoderm to differentiate into the neural plate. If these
cells also receive signals from FGF, they will differentiate into the
spinal cord; in the absence of FGF the cells become brain tissue.
References:
1.
Chen, D. et al., Growth Factors 22:233, 2004.
2.
Aberdam, D. et al., Bull. Acad. Natl. Med. 189:645, 2005.
3.
Czyz, J. & Wobus, A., Differentiation 68:167, 2001.
|
|
|
Specific
detection of NSO-cell expressed recombinant human BMP-4 proteins in
Western blot analysis using BMP-4
Monoclonal Antibody (25H14).
|
|
