| Cat.# |
CB12146
|
| Size |
90
µg / 200
ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
recombinant human Aurora Kinase B protein
|
|
Species
&
specficity:
|
Anti-AurkB
specifically detects endogenous AurkB protein. The molecular
weight of AurkB is 41 kDa. Anti-AurkB reacts with human,
mouse, & rat AurkB. Anti-AurkB does not cross-react with
other Aurora Kinase family proteins.
|
| Storage: |
Store
at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw
cycles.
|
| MW:
|
41
kDa
|
|
Application:
|
WB
|
|
Dilution:
|
1:1000
|
|
Background:
Chromosomal
segregation during mitosis as well as meiosis is regulated by kinases
and phosphatases. The Aurora kinases associate with microtubules during
chromosome movement and segregation. Aurora kinase B (AurkB) localizes
to microtubules near kinetochores, specifically to the specialized
microtubules called K-fibers. AurkB functions in the attachment of the
mitotic spindle to the centromere1. In cancerous cells, over
expression of these enzymes cause unequal distribution of genetic
information creating aneuploidy cells, a hallmark of cancer. Thus, AurkB
is emerging as a novel drug target for cancer treatment2, 3.
References:
1.
Shannon, K. B. & Salmon,
E.D.: Curr Biol.
12:R458, 2002.
2.
Mahadevan, D. et al.: Curr Med
Chem Anticancer Agents 3:25, 2003.
3.
Vischioni,
B. et al.: Mol. Cancer Ther. 5:2905, 2006.
|
|
|
