| Cat.# |
CB0536
|
| Size |
50
µg / 200 ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
recombinant
human
Noggin
protein |
| Species: |
human |
| Storage: |
-20°
|
| MW:
|
27
kDa
|
|
Application:
|
WB
|
| Dilution:
|
1:1000
|
|
Background:
The gene noggin,
originally cloned in Xenopus, encodes a secreted factor expressed in the
Spemann organizer, where it functions to oppose the ventralizing
influence of bone morphogenetic proteins (BMPs). Noggin protein acts by
binding directly to BMPs, thereby preventing them from interacting with
their receptors.The interaction between bone morphogenetic proteins (BMPs)
and Noggin, is critical for normal development1. Recently, it
was shown that Noggin and bFGF cooperate to maintain the pluripotency
of human embryonic stem cells in the absence of feeder layers. This feeder-free culture system will provide a more
reliable alternative for future therapeutic applications of hES cells2.
References:
1.
MacMahon,
J. A. et al., Gene Dev 12:1438, 1998.
2.
Wang,
G. et al., Biochem
Biophys Res Commun 330:934, 2005.
|
|
Detection
of human Noggin proteins by Western blot with anti-Noggin (11A6)
monoclonal antibody.
|
