Anti-Src monoclonal antibody

Src: Monoclonal Anti-Src Antibody (10H4)

Cat.#

CB0368

Size

60 µg / 200 ml

Isotype:

mouse monoclonal IgG1

Epitope:

Sf9 cell-expressed recombinant human c-Src proteins.

Specificity & sensitivity:

human, mouse, rat, monkey

(This antibody specifically detects endogenous levels of Src proteins. This antibody does not cross-react with other Src-family members by Western blot analysis using specific recombinant proteins.)

Storage:

-20°

MW:

60 kDa

Application:	WB
Dilution:	1:1000

Background: Src was the first protooncogene described and was among the first molecules in which tyrosine kinase activity was documented. The Src gene family has nine known members (Blk, c-Fgr, Fyn, Hck, Lck, Lyn, c-Src, c-Yes and Yrk), each encoding a cytoplasmic protein-tyrosine kinase (PTK) believed to be involved in signal transduction. The Src PTKs contain three domains (SH1, SH2 and SH3) that are found in many other signalling proteins. The SH1 domain has PTK activity, whilst the SH2 and SH3 domains are involved in mediating protein-protein interactions by binding to phosphotyrosine-containing and proline-rich motifs, respectively. The expression patterns of the c-Src PTKs suggest that they function in a broad range of biological situations, in many cases regulating the behaviour of terminally-differentiated, post-mitotic cell types¹. Src has been defined as a common modular structure that participates in much of the crosstalk between the cytoplasmic protein tyrosine kinases and tyrosine kinase receptors². Src has been found to be overexpressed and activated in a large number of different cancers. Cellular Src has been shown to activate a number of different effectors that are involved in different aspects of cancer biology such as metastasis, cell cycle regulation and cell survival^{3,
4}. Cumulative data mark Src signaling as attractive therapeutic targets in different cancers.

References:

1. Homsi, J. et al., Expert Opin Ther Targets. 11:91-100, 2007.

2. Brickell, P. M. et al., Crit Rev Oncog 3:401, 1993.

3. Bolen, J. B. et al., FASEB J 6:3403, 1992.

4. Alvarez, R. H. et al., Cancer 107:1918, 2006.

anti-Src antibody western blot

Various cell lysates were subjected for Western blot analysis using Src (10H4) monoclonal antibody. A 60 kDa Src protein band was specifically detected in all cell lysates.