| Cat.# |
CB0328
|
| Size |
60
µg / 200
ml |
| Isotype:
|
Rabbit IgG1
|
| Epitope: |
Synthetic peptide corresponding to C-terminal sequence of human histone
H3 proteins
|
|
Species
&
specficity:
|
Human,
mouse, & rat histone
H3
|
| Storage: |
Store
at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw
cycles.
|
| MW:
|
19
kDa
|
|
Application:
|
WB
|
IP |
IHC (Paraffin) |
|
Dilution:
|
1:1000
|
1:50 |
1:50 |
|
Background:
Histone
H3 is one of the four histones, along with H2A, H2B and H4, which form
the eukaryotic nucleosome octamer core; the nucleosome octamer winds
~146 DNA base-pairs. It is a highly conserved protein of 135 amino acid
residues. The N-terminal tail of histone H3 protrudes from the globular
nucleosome core and can undergo several different types of epigenetic
modifications that influence cellular processes. These modifications
include the covalent attachment of methyl groups to arginine, methyl or
acetyl groups to lysine, and phosphorylation of serine or threonine
residues. Methylation of lysine9 has been associated with gene silencing1.
Acetylation of histone H3 occurs at several different lysine positions
in the histone tail and is performed by a family of enzymes known as
Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly
seen in genes that are being actively transcribed into RNA2.
Phosphorylation of histone H3 at serine 10 occurs during mitosis and
meiosis in a wide range of eukaryotes and has been shown to be required
for proper chromosome transmission3.
References:
1.
Schneider, R et al:
Nature Cell Biol. 6:73, 2004.
2.
Liang, G. et al.: Proc. Natl. Acad. Sci. USA 101:7357, 2004.
3.
Hsu, J. Y. et al.: Cell 102:279, 2000.
|
|
|
