Chicken Aortic Smooth Muscle Cells

(CAOSMC)

Chicken Aortic Smooth Muscle Cells (CAOSMC) are primary cells derived from fibrous-plaque-free thoracic aorta of adult chicken. They are cryopreserved at second passage and can be propagated at least 12 population doublings. CAOSMC may be used for the investigation of atherosclerosis ¹ and acute vascular injury. They have been used for studying smooth muscle cell proliferation, hypertrophy, and secretion of extracellular matrix components^2,3.

Ross, R., Nature 362:801 (1993).
Fuster, V. et al, NEJM 326:242 (1992).
Fisher, D. Z., personal communication (1997).

Animal Cells - Chicken Aortic Smooth Muscle Cells

Characterization: Positive for smooth muscle cell specific a -actin expression.

Product Name	Product Description	Size (vol)	Cat. No.
CAOSMC Total Kit	Each kit contains an ampoule of cryopreserved CAOSMC (C354-05), 500 ml of Chicken SMC Growth Medium (C311-500), and a Subculture Reagent Kit (090K).	1 Kit	C354K-05
Cryopreserved CAOSMC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Chicken SMC Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	C354-05
Proliferating CAOSMC In Flasks	Cultured and shipped in flasks at third passage in Chicken SMC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	C355-25 C355-50 C355-75
Proliferating CAOSMC In Multiwell Plates	Cultured and shipped in flasks at third passage in Chicken SMC Growth Medium.	6 well 12 well 24 well 48 well 96 well	C355-6W C355-12W C355-24W C355-48W C355-96W
Chicken SMC Growth Medium	Optimized and ready for use	500 ml	C311-500
Chicken SMC Growth Medium Kit		Yields 500 ml	C311K-500
Chicken SMC Basal Medium	Basal Medium & Growth Supplement packaged separately	500 ml	C310-500
Chicken SMC Growth Supplement		50 ml	C311-GS
Chicken SMC Differentiation Medium	Add Growth Supplement before use	250 ml	C311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution		090K