| Cat.# |
CA10310 |
| Size |
160
µg / 200 ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
recombinant
human hnRNP-A2/B1 protein |
| Species: |
human |
| Storage: |
Store at 4°
for frequent use; at -20°
for at least one year |
| MW:
|
34
& 36 kDa
|
|
Application:
|
WB
|
| Dilution:
|
1:1000
|
|
Background:
The
heterogeneous nuclear ribonucleoproteins (hnRNPs) are a large family of
nucleic acid binding proteins that are often found in, but not
restricted to, the 40S-ribonucleoprotein particle. Subsets of hnRNPs are
strictly nuclear while others shuttle between the nucleus and cytoplasm1. HnRNP complexes are also major constituents of the spliceosome.
They are composed of approximately 30 different proteins which can bind
to nascent pre-mRNA. . Members of the hnRNP family have been implicated
to have roles in many aspects of mRNA maturation/turnover and in
telomere and telomerase regulation.
Among these, the hnRNP-A/B proteins form a subgroup of highly
related proteins consisting of two adjacent RNA binding domains (RBD)
within the N-terminal parts, whereas the C-terminal halves contain
almost 50% glycine residues2. HnRNP A2/B1 has been found
overexpressed in the early stage of lung cancer and other cancers3,4. Thus, hnRNP-A2/B1 can be a marker of
carcinogenesis.
References:
1.
Ford,
LP et al.,
Oncogene
21:580 (2002).
2.
Steiner,
G et al.,
Mol Biol Rep 23:167 (1996).
3.
Zhou,
J. et al., Lung Cancer. 34:341(2001).
4.
Zhou, J. et al., Breast Cancer Res Treat. 66:217 (2001).
|
|
Specific
detection of hnRNP-A2/B1 proteins from various cell lysates in Western
blot analysis using hnRNP-A2/B1 Monoclonal Antibody (8G26).
|
