| Cat.# |
CA10249 |
| Size |
100
µg / 200 ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
recombinant
human Zap70 protein |
| Species: |
human,
mouse |
| Storage: |
Store at 4°
for frequent use; at -20°
for at least one year |
| MW:
|
70
kDa
|
|
Application:
|
WB
|
| Dilution:
|
1:1000
|
|
Background:
ZAP-70
is a protein tyrosine kinase (PTK) required for T-cell development and
T-cell antigen receptor (TCR) function. ZAP-70 is associated with the
phosphorylated antigen receptor and undergoes tyrosine phosphorylation
following receptor activation. It was demonstrated that tyrosine
phosphorylation of ZAP-70 resulted in an increase in its catalytic
activity and that this activation is mediated by the phosphorylation of
tyrosine residue 493 by the src family of PTKs1. Its key role
in thymocytes development and mature T lymphocytes activation has been
illustrated by the characterization of several human immunodeficiencies
presenting with mutations in the zap-70 gene resulting in the absence of
ZAP-70 expression. More recently, it has been shown that deregulation of
ZAP-70 activity can induce autoimmune diseases. Finally, ZAP-70
expression has been shown in some B chronic lymphocytic leukaemia and
correlated with bad prognosis of the disease. The diversity of
pathologies associated with deregulation of ZAP-70 demonstrates its key
role in immune responses. Research aiming at deciphering the different
signalling pathways regulated by ZAP-70 will not only shed some lights
on these pathologies, but will also help finding new pharmacological
tools, targeting ZAP-70, designed to induce immunosuppression or
tolerance2.
References:
1.
Chan,
AC et al. : EMBO J. 14:2499 (1995).
2.
Hivroz,
C., Med Sci (Paris). 21:150 (2005).
|
|
Specific
detection of Zap-70 proteins from Jurkat and CTLL-2 cell lysates in
Western blot analysis using Zap-70 Monoclonal Antibody (3D18).
|
