| Cat.# |
CA10247 |
| Size |
120
µg / 200 ml |
| Isotype:
|
mouse
monoclonal IgG1
|
| Epitope: |
recombinant
human SRC-3/ AIB-1 protein N-terminal fragments (1-250 aa) |
| Species: |
human |
| Storage: |
Store at 4°
for frequent use; at -20°
for at least one year |
| MW:
|
160
kDa
|
|
Application:
|
WB
|
| Dilution:
|
1:1000
|
|
Background:
Nuclear
hormone receptors are ligand-dependent transcription factors that
require coactivators to regulate target gene expression. The steroid
receptor coactivator-3 (SRC-3), also known as p/CIP, RAC3, AIB1, ACTR
and TRAM-1, is a cancer-amplified coactivator in the SRC gene family
that also contains SRC-1 and SRC-2. SRC-3 interacts with nuclear
receptors and certain other transcription factors, recruits histone
acetyltransferases and methyltransferases for chromatin remodeling and
facilitates target gene transcription1. SRC-3 phosphorylation
and methylation have been shown to regulate such coactivator complex
assembly2,3. Accumulated
results from both ex vivo and animal model studies indicate that SRC-3
plays important roles in many biological processes involving cell
proliferation, cell migration, cell differentiation, somatic growth,
sexual maturation, female reproductive function, and vasoprotection1.
Moreover, SRC-3 has been associated with multiple cancers, including
breast, gastric and prostate cancers4.
References:
1.
Liao,
L. et al. : J Steriod Biochem Mol Biol 83:3, 2002
2.
Feng,
Q. et al.: Mol Cell Biol 26:7846, 2006
3.
Wu,
H. et al. : J Biol Chem 281:21848, 2006
4.
Yan, J. et al. : Acta
Pharmacol Sin 27:387, 2006
|
|
Specific detection of SRC-3/AIB-1 proteins from MCF7 and T47D breast
cancer cell lysates in Western blot analysis using SRC-3/AIB-1
Monoclonal Antibody (14H6).
|
