Bovine Renal Artery Smooth Muscle Cells

(BRASMC)

Bovine Renal Artery Smooth Muscle Cells (BRASMC) are derived from tunica intima and tunica media of normal bovine renal arteries. They are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings.

Studies show cyclosporine-induced renal artery smooth muscle contraction is associated with increases in the phosphorylation of specific contractile regulatory proteins¹. The renal artery smooth muscle is uniquely refractory to angiotensin stimulation and may be protective in the role of the kidney in regulating blood pressure through the renin-angiotensin system².

Beall, A. et al, Biochem Biophys Acta., 1449(1):41-9 (1999).
Whitney, E.G. et al, J. Surg. Res. 61(2):307-10 (1996).

Animal Cells - Bovine Renal Artery Smooth Muscle Cells

Characterization: Positive for smooth muscle specific a-actin expression.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
BRASMC Total Kit	Each kit contains an ampoule of cryopreserved BRASMC (B950-05), 500 ml of Bovine Smooth Muscle Cell Growth Medium (B311-500), and a Subculture Reagent Kit (090K).	1 Kit	B950K-05
Cryopreserved BRASMC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Bovine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	B950-05
Proliferating BRASMC In Flasks	Cultured and shipped in flasks at third passage in Bovine Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	B951-25 B951-50 B951-75
Proliferating BRASMC In Multiwell Plates	Cultured and shipped in plates at third passage in Bovine Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	B951-6W B951-12W B951-24W B951-48W B951-96W
Bovine SMC Growth Medium	Optimized and ready for use	500 ml	B311-500
Bovine SMC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	B311K-500
Bovine SMC Basal Medium	Add Growth Supplement before use	500 ml	B310-500
Bovine SMC Growth Supplement		50 ml	B311-GS
Bovine SMC Differentiation Medium		250 ml	B311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K