Bovine Renal Artery Endothelial Cells

(BRAEC)

Bovine Renal Artery Endothelial Cell (BRAEC) are derived from renal artery of USDA inspected cattle. They are cryopreserved at the second passage and can be propagated at least 10 doublings. Endothelial cell dysfunction is the primary cause of no reflow phenomenon in postischemic kidneys¹. Low endothelial nitric oxide synthasae activity may contribute in hypertension^2,3 in renal failure patients. BRAEC have been used as a cellular model for studying the pathogenesis of septic shock⁴.

1. Brodsky, S.V. et al, Am. J. Physiol Renal Physiol. 282(6):F1140 (2002).

2. Goonasekera, C.D. et al, Pediatr. Nephrol. 12(8):676 (1998).

3. Xioa, S. et al, Acta Physiol. Scand. 168(1):175 (2000).

4. Lama, S. et al, Am. J. Physiol. 261(4 pt 1):C634 (1991).

Animal Cells - Bovine Renal Artery Endotheilial Cells

Characterization: Positive for DiI-Ac-LDL uptake.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
BRAEC Total Kit	Each kit contains an ampoule of cryopreserved BRAEC (B900-05), 500 ml of Bovine Endothelial Cell Growth Medium (B211-500), and a Subculture Reagent Kit.	1 Kit	B900K-05
Cryopreserved BRAEC	(>500,000 cells in an ampoule) Cryopreserved at first passage in Bovine Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	B900-05
Proliferating BRAEC In Flasks	Cultured and shipped in flasks at second passage in Bovine Endothelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	B901-25 B901-50 B901-75
Proliferating BRAEC In Multiwell Plates	Cultured and shipped in flasks at second passage in Bovine Endothelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	B901-6W B901-12W B901-24W B901-48W B901-96W
Bovine Endothelial Cell Growth Medium	Optimized and ready for use	500 ml	B211-500
Bovine Endothelial Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	B211K-500
Bovine Endothelial Cell Basal Medium	Add Growth Supplement before use	500 ml	B210-500
Bovine Endothelial Cell Growth Supplement		50 ml	B211-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K