Bovine Endometrial Epithelial Cells

(BEnEpC)

Bovine Endometrial Epithelial Cells (BEnEpC) are isolated from epithelium of the bovine endometrium. The cells are cryopreserved at second passage. BEnEpC can be cultured and propagated for at least 10 population doublings. BEnEpC are commonly used with Bovine Endometrial Stromal Cells as an in vitro model for studying maternal recognition of pregnancy, such as the effects of IFN-t and oxytocin,¹^,2,3 the effects of prostaglandins on the maintenance, or lack thereof, of pregnancy,^3,4 and the effects of estradiol and progesterone.⁵ In addition, it has been shown that BEnEpC can maintain embryo development to blastocyte stage as effectively as oviductal cells.⁶

Desnoyers, G.D. et al, Reprod. Fertil. Dev. 6:193-202 (1994)
Meyer, M.D. et al, J. Dairy Sci. 79:1375-1384 (1996)
Horn, S. et al, Hum. Reprod. Update. 4(5):605-614 (1998)
Asselin, E. & Fortier, M.A., J. Cell Physiol. 168(3):600-607 (1996)
Xiao, C.W. et al, Endocrinology 139(5):2293-2299 (1998)
Goff, A.K. & Smith, L.C., Theriogenalogy 49(5):1021-1030 (1998)

Characterization: Positive for DiI-Ac-LDL uptake.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
BEnEpC Total Kit	Each kit contains an ampoule of cryopreserved BEnEpC (B932-05), 500 ml of BEnEpC Growth Medium (B911-500), a Subculture Reagent Kit (090K).	1 Kit	B932K-05
Cryopreserved BEnEpC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Bovine Endometrial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	B932-05
Proliferating BEnEpC In Flasks	Cultured and shipped in flasks in BEnEpC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	B933-25 B933-50 B933-75
Proliferating BEnEpC In Multiwell Plates	Cultured and shipped in flasks in BEnEpC Growth Medium.	6 well 12 well 24 well 48 well 96 well	B933-6W B933-12W B933-24W B933-48W B933-96W
BEnEpC Growth Medium	Optimized and ready for use	500 ml	B911-500
BEnEpC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500ml	B911K-500
BEnEpC Basal Medium	Add Growth Supplement before use	500 ml	B910-500
BEnEpC Growth Supplement		50 ml x 2	B911-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K