Bovine Brain Microvascular Endothelial Cells

(BBMVEC)

Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings. Brain Microvascular Endothelial Cells form the basis of the blood-brain barrier, and have been used as a model for research on bacterial meningitis¹^,2,3. Hypoxia-ischemia studies showed that BBMVEC cell death may exacerbate ischemic brain injury⁴. BBMVEC have also been used to show oxyhemoglobin induces apoptosis in vascular endothelial cells⁵. Co-culture with Atrocytes generate an in vitro model that closely mimics the in vivo situation for evaluating drug transport⁶.

Zysk, G. et al, Infect. Immun. 69(2):845-852 (2001)
Prasadarao, N.V. et al, Infect. Immun. 67(3):1131-1138(1999)
Wang, Y. et al, Infect. Immun. 67(9):4751-4756 (1999)
Zhang, J. et al, Brain Res. 877(2):134-140 (2000)
Meguro, T. et al, J. Neurochem. 77(4):1128-1135
Cecchelli, R. et al, Adv. Drug Deliv Rev 36:165 (1999)

Animal Cells - Bovine Brain Microvascular Endothelial Cells

Characterization: Positive for DiI-Ac-LDL uptake.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
BBMVEC Total Kit	Each kit contains an ampoule of cryopreserved BBMVEC (B840-05), 500 ml of BBMVEC Growth Medium (B811-500), and a Subculture Reagent Kit (090K).	1 Kit	B840K-05
Cryopreserved BBMVEC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Bovine Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	B840-05
Proliferating BBMVEC In Flasks	Cultured and shipped in flasks at third passage in BBMVEC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	B841-2 B841-50 B841-75
Proliferating BBMVEC In Multiwell Plates	Cultured and shipped in plates at third passage in BBMVEC Growth Medium.	6 well 12 well 24 well 48 well 96 well	B841-6W B841-12W B841-24W B841-48W B841-96W
BBMVEC Growth Medium	Optimized and ready for use	500 ml	B819-500
BBMVEC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	B819K-500
BBMVEC Basal Medium	Add Growth Supplement before use	500 ml	B818-500
BBMVEC Growth Supplement		50 ml x 2	B819-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution)	100 ml	090K
BBMVEC RNA	Total RNA prepared from BBMVEC	10 µg 25 µg	B840-R10 B840-R25